Anti-GCP6 antibody (bs-13321R)

Anti-GCP6 antibody

DATASHEET

Host:Rabbit

Target Protein:GCP6

IR:Immunogen Range:651-750/1819

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:85378

Swiss Prot:Q96RT7

Source:KLH conjugated synthetic peptide derived from human GCP6:651-750/1819 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The g-Tubulin complex is composed of g Tubulin and the g-Tubulin complex-associated proteins GCP2, GCP3, GCP4, GCP5 and GCP6, all of which are essential components of microtubule organizing centers. g-Tubulin complex components are localized to both the centrosome, where they are involved in microtubule nucleation, and to the cytoplasm, where they exist as soluble complexes that can be recruited to the centrosome as needed. Although the GCP proteins are related, they have distinct roles which contribute to the proper function of the g-Tubulin complex. GCP6 (g-Tubulin complex component 6), also known as TUBGCP6, localizes to the centrosome and is a ubiquitously expressed 1,819 amino acid member of the g-Tubulin complex. Unlike GCP3 and GCP2, GCP6 is not well conserved among eukaryotes. Three isoforms of GCP6 exist due to alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCP6) Polyclonal Antibody, Unconjugated (bs-13321R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.