Anti-GFI1 antibody (bs-13337R)

Anti-GFI1 antibody

DATASHEET

Host:Rabbit

Target Protein:GFI1

IR:Immunogen Range:301-400/422

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2672

Swiss Prot:Q99684

Source:KLH conjugated synthetic peptide derived from human GFI1/ZNF163:301-400/422 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Growth factor independent 1 (Gfi-1) is a transcriptional repressor that specifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA. The carboJNC-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site. Gfi-1 also contains a 20 amino acid SNAG domain which mediates transcriptional repression. It represses Bax at the mRNA and protein levels, resulting in the inhibition of cell death. Gfi1 is expressed outside the lymphoid system in granulocytes and activated macrophages. Gfi-1B, a related protein, is a transcriptional repressor primarily expressed in bone marrow and spleen. Gfi-1B is a direct repressor of the p21 promoter and the Socs 1 and 3 promoters. The genes encoding human Gfi-1 and Gfi-1B map to chromosome 1p22 and 9q34.3, respectively.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
Sheep
Xenopus laevis.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lung (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- GFI1 (bs-13337R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD