Anti-IFN-Alpha antibody (bs-8995R)

Anti-IFN-Alpha antibody

DATASHEET

Host:Rabbit

Target Protein:IFN-Alpha

IR:Immunogen Range:121-189/189

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:15962

Swiss Prot:P01572

Source:KLH conjugated synthetic peptide derived from mouse IFN-Alpha/IFNA1/IFNA13/Interferon alpha 1:121-189/189 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The genes encoding type I interferons (IFNs), which include 14 IFN-alphagenes (two of which are IFN-alpha1 and IFN-alpha13), one IFN-∫ gene, one IFN-w (also known as IFN-alpha II1) gene and a number of IFN-w pseudogenes, are clustered on human chromosome 9. IFN-alpha and -beta are cytokines that are widely known to induce potent antiviral activity. They exert a variety of other biological effects, including antitumor and immunomodulatory activities and are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases. IFN-w is antigenically different from human IFN-alpha, IFN-beta or IFN-gamma, but is a component of natural mixtures of IFN species produced by virus-induced leukocytes or Burkitt's lymphoma cells. The type I interferon receptor (IFN-alphaR) interacts with IFN-alpha, IFN-beta and IFN-w, and seems to be a multisubunit receptor.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: mouse intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IFN-Alpha Polyclonal Antibody, Unconjugated(bs-8995R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei