Anti-CD39 antibody (bs-15383R)

Anti-CD39 antibody

DATASHEET

Host:Rabbit

Target Protein:CD39

IR:Immunogen Range:101-200/510

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:953

Swiss Prot:P49961

Source:KLH conjugated synthetic peptide derived from human CD39/entpd1 :101-200/510 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Members of the ecto-nucleoside triphosphate diphosphohydrolase (eNTPDase) protein family are glycosylated enzymes that hydrolyze nucleoside 5’-triphosphates and 5’-diphosphates in extracellcular space. The enzymatic activities of eNTPDase are dependent on cations such as magnesium and calcium. Members of this protein family differ in their affinities for triphosphates versus diphosphates as substrate material. CD39L3, also known as Ectonucleoside triphosphate diphosphohydrolase 3 or NTPDase 3, is a 529 amino acid multi-pass membrane protein that has a three-fold preference for hydrolysis of ATP over ADP. Both calcium and magnesium serve as cofactors for CD39L3’s extremely active extracellular nucleotide hydrolysis site. CD39L3 is expressed in spleen, prostate, brain and pancreas, though moderate to low expression is observed in most tissues, with the exception of liver and peripheral blood leukocytes.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control: Raji cells (blue).
Primary Antibody:Rabbit Anti- CD39 antibody(bs-15383R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ;
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-15383R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.