Anti-HAPLN3 antibody (bs-15411R)

Anti-HAPLN3 antibody

DATASHEET

Host:Rabbit

Target Protein:HAPLN3

IR:Immunogen Range:51-150/360

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:145864

Swiss Prot:Q96S86

Source:KLH conjugated synthetic peptide derived from human HAPLN3:51-150/360 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:HAPLN3 is a 360 amino acid protein encoded by the human gene HAPLN3. HAPLN3 belongs to the HAPLN family and contains 1 Ig-like V-type (immunoglobulin-like) domain and two Link domains. HAPLN3 mediates the binding of complexes containing hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. HAPLN3 may also be involved in the formation of extracellular matrices contributing to perineuronal nets and facilitate the understanding of a functional role of these extracellular matrices. HAPLN3 is widely expressed with highest levels in spleen and placenta.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Horse
Chimpanzee
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-HAPLN3 antibody (bs-15411R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.