Anti-HBQ1 antibody
英文名称 | HBQ1 |
中文名称 | 血红蛋白theta亚型1抗体 |
别 名 | HBAT_HUMAN; HBQ1; Hemoglobin subunit theta 1; Hemoglobin subunit theta-1; Hemoglobin theta 1; Hemoglobin theta 1 chain; Hemoglobin theta-1 chain; Theta 1 globin; Theta-1-globin; |
DATASHEET
Host:Rabbit
Target Protein:HBQ1
IR:Immunogen Range:1-100/142
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3049
Swiss Prot:P09105
Source:KLH conjugated synthetic peptide derived from human HBQ1:1-100/142
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Theta-globin mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue. The theta-1 gene may be expressed very early in embryonic life, perhaps sometime before 5 weeks. Theta-1 is a member of the human alpha-globin gene cluster that involves five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-2 -pseudoalpha-1 - alpha-2 - alpha-1 - theta-1 - 3'. Research supports a transcriptionally active role for the gene and a functional role for the peptide in specific cells, possibly those of early erythroid tissue.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
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For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HBQ1) Polyclonal Antibody, Unconjugated (bs-15419R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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