Anti-phospho-SLP76 (Tyr113) antibody
| 英文名称 | phospho-SLP76 (Tyr113) |
| 中文名称 | 磷酸化淋巴细胞胞浆蛋白2抗体 |
| 别 名 | SLP76 (phospho Y113); p-SLP76 (Y113); p-LCP2; 76 kDa tyrosine phosphoprotein; CG8697; LCP 2; LCP 2; LCP2; LCP2_HUMAN; Lymphocyte Cytosolic Protein 2; Lymphocyte Cytosolic Protein 2; SH2 Domain Containing Leukocyte Protein 76 KD; SH2 domain containing leukocyte protein of 76kD; SH2 domain containing leukocyte protein of 76kDa; SH2 domain-containing leukocyte protein of 76 kDa; SLP 76; SLP 76; SLP 76 tyrosine phosphoprotein; SLP-76 tyrosine phosphoprotein; SLP76; SLP76 tyrosine phosphoprotein. |
DATASHEET
Host:Rabbit
Target Protein:phospho-SLP76 (Tyr113)
IR:Immunogen Range:DD(p-Y)ES
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3937
Swiss Prot:Q13094
Source:KLH conjugated Synthesised phosphopeptide derived from human SLP76 around the phosphorylation site of Tyr113:DD(p-Y)ES
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The translational product of the Vav proto-oncogene is exclusively expressed in cells of hematopoietic origin and is critical for lymphocyte development and activation. However, the biochemical basis of Vav’s function is unclear. Vav contains a single SH2 domain that is required for its association with the T cell receptor (TCR). Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production. When co-expressed, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response. Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, serine/threonine or lipid kinase families, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1. The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Cross Reactive Species:Human
Mouse
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-phospho-SLP76 (Tyr113) (bs-13663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 75 kD
Observed band size: 75 kD
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