Anti-PCDH10 antibody (bs-13723R)

Anti-PCDH10 antibody

DATASHEET

Host:Rabbit

Target Protein:PCDH10

IR:Immunogen Range:21-120/1040

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:57575

Swiss Prot:Q9P2E7

Source:KLH conjugated synthetic peptide derived from human PCDH10:21-120/1040 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDH10 (protocadherin 10), also known as PCDH19 or OL-PCDH, is a 1,040 amino acid single-pass type I membrane protein that contains six cadherin domains and one transmembrane domain. Expressed at moderate levels in brain, ovary and testis and present at lower levels in all other tissues, PCDH10 functions as a calcium-dependent cell-adhesion protein that may function as a tumor suppressor. When underexpressed, PCDH10 is associated with the progression of various carcinomas, including gastric cancer.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Rat
Pig
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCDH10) Polyclonal Antibody, Unconjugated (bs-13723R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.