Anti-GEP100/IQSEC1 antibody (bs-13960R)

Anti-GEP100/IQSEC1 antibody

DATASHEET

Host:Rabbit

Target Protein:GEP100/IQSEC1

IR:Immunogen Range:451-550/963

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9922

Swiss Prot:Q6DN90

Source:KLH conjugated synthetic peptide derived from human GEP100:451-550/963 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF6 plays a role in protein trafficking near the plasma membrane, including receptor recycling, cell adhesion and cell migration. ARF6 colocalizes with the ARF guanine nucleotide-exchange protein (GEP) BRAG2, also designated GEP100. BRAG2 is ubiquitously expressed as two isoforms, BRAG2a and BRAG2b, which can cycle between the cytoplasm and the nucleus. BRAG2, via its interaction with ARF6, is involved in the regulation of cell adhesion by controlling Integrin ∫1 endocytosis and E-cadherin redistribution. BRAG2 has also been shown to bind directly to Tyr1068/1086-phosphorylated EGFR to activate ARF6, which induces tumor invasion in MCF7 cells. Therefore, BRAG2 may contribute to the metastasis and malignancy of some breast cancer cells.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- GEP100/IQSEC1 (bs-13960R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 108 kD
Observed band size: 108 kD