Anti-phospho-cGKI (Thr515) antibody (bs-7368R)

Anti-phospho-cGKI (Thr515) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-cGKI (Thr515)

IR:Immunogen Range:KK(p-T)WT

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5592

Swiss Prot:Q13976

Source:KLH conjugated Synthesised phosphopeptide derived from human cGKI around the phosphorylation site of Thr515:KK(p-T)WT 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PKG plays an important stimulatory role in platelet activation.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
JEG-3(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-cGKI (Thr515) (bs-7368R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kD
Observed band size: 74 kD