Anti-PIAS4/PIASy antibody (bs-14480R)

Anti-PIAS4/PIASy antibody 

DATASHEET

Host:Rabbit

Target Protein:PIAS4/PIASy

IR:Immunogen Range:21-120/510

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:51588

Swiss Prot:Q8N2W9

Source:KLH conjugated synthetic peptide derived from human PIASy:21-120/510 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The IL-6-type family of cytokines, which includes IL-6 and a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the STAT family are involved in IL-6 family-mediated signal transduction pathways, and upon activation undergo phosphorylation, dimerization and translocation to the nucleus. The duration and intensity of a cell's response to cytokines can be adjusted by the effect of several regulatory mechanisms. One example involves the protein inhibitor of activated signal transducer and activator of transcription (STAT) family (PIAS family) of proteins, which act as negative regulators of STATs in cytokine signaling. PIAS proteins are able to coactivate steroid receptor-dependent transcription as well. Human PIASy is a 510 amino acid transcriptional corepressor of the androgen receptor (AR). In addition, PIASy may regulate p53-mediated events and may direct p53 into a transactivation-independent mode of apoptosis.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIAS4) Polyclonal Antibody, Unconjugated (bs-14480R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.