Anti-EEFSEC antibody (bs-14505R)

Anti-EEFSEC antibody 

DATASHEET

Host:Rabbit

Target Protein:EEFSEC

IR:Immunogen Range:21-120/596

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:60678

Swiss Prot:P57772

Source:KLH conjugated synthetic peptide derived from human EEFSEC:21-120/596 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:EEFSEC is a 596 amino acid protein that localizes to both the nucleus and the cytoplasm and belongs to the GTP-binding elongation factor family. Functioning as a translation factor, SELB binds GTP and GDP and is necessary for the incorporation of selenocysteine into target proteins. The gene encoding SELB maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EEFSEC) Polyclonal Antibody, Unconjugated (bs-14505R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.