Anti-FRY/C13orf14 antibody (bs-16183R)

Anti-FRY/C13orf14 antibody

DATASHEET

Host:Rabbit

Target Protein:FRY/C13orf14

IR:Immunogen Range:101-200/3013

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10129

Swiss Prot:Q5TBA9

Source:KLH conjugated synthetic peptide derived from human FRY/C13orf14:101-200/3013 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:In yeast, flies, and worms, the Dbf2-related (Ndr) kinase protein family functions in various aspects of cell polarity and morphogenesis. The Drosophila melanogaster protein, furry, is responsible for maintaining integrity of polarized cell extensions, such as epidermal hair cells, lateral extensions of the arista and the shafts of neuronal sensory bristles. Mutations in furry lead to the formation of branched arista laterals, bristles and hairs. The yeast homolog of furry, Mor2, is important for the localization of F-actin specifically at the cell ends and is required for the restriction of the growth zones. The mammalian homolog of the Drosophila furry protein is FRY, also known as C13orf14, a 3,013 amino acid protein that probably functions as a transcription factor for genes that regulate the actin cytoskeleton. The gene encoding FRY maps to chromosome 13, which comprises nearly 4% of human DNA and contains around 114 million base pairs and 400 genes.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell:human hepatocellular carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FRY/C13orf14 Polyclonal Antibody, Unconjugated(bs-16183R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining