Anti-ZNF683 antibody (bs-16521R)

Anti-ZNF683 antibody 

DATASHEET

Host:Rabbit

Target Protein:ZNF683

IR:Immunogen Range:341-440/524

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:257101

Swiss Prot:Q8IZ20

Source:KLH conjugated synthetic peptide derived from human ZNF683:341-440/524 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Zinc finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zinc finger protein 683 (ZNF683) is a 509 amino acid member of the Krüppel C2H2-type zinc finger protein family. Localized to the nucleus, ZNF683 contains four C2H2-type zinc fingers through which it is thought to be involved in DNA-binding and transcriptional regulation. Two isoforms of ZNF683 exist as a result of alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF683) Polyclonal Antibody, Unconjugated (bs-16521R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.