Anti-phospho-HSF1 (Ser303 + Ser307) antibody (bs-16556R)

Anti-phospho-HSF1 (Ser303 + Ser307) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-HSF1 (Ser303 + Ser307)

IR:Immunogen Range:PP(p-S)PPQ(p-S)PR

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3297

Swiss Prot:Q00613

Source:KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303:PP(p-S)PPQ(p-S)PR 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSF1(Ser303 + Ser307)) Polyclonal Antibody, Unconjugated (bs-16556R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.