Anti-DNA Polymerase delta, catalytic subunit antibody
| 英文名称 | DNA Polymerase delta, catalytic subunit |
| 中文名称 | DNA聚合酶δ催化亚单位/DNA pol δ cat抗体 |
| 别 名 | CDC2; CDC2 homolog; DNA directed DNA polymerase delta 1; DNA directed polymerase delta 1; DNA pol delta 1; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; DPOD1_HUMAN; POLD; POLD 1; POLD1; Polymerase (DNA directed) delta 1 catalytic subunit 125kDa; Polymerase DNA directed delta 1 catalytic subunit 125kD. |
DATASHEET
Host:Rabbit
Target Protein:DNA Polymerase delta, catalytic subunit
IR:Immunogen Range:751-850/1107
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5424
Swiss Prot:P28340
Source:KLH conjugated synthetic peptide derived from human DNA Polymerase delta, catalytic subunit:751-850/1107
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). Exonucleases involved in these processes include DNA polymerases, including DNA pol ? and é. DNA pol ? consists of two subunits, p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50 (3,4). DNA pol ? can be regulated by cell cycle proteins (5). DNA pol é is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene (6,7). The exact reactions catalyzed by DNA pol ? and é on leading and lagging strands have not yet been elucidated.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDC2) Polyclonal Antibody, Unconjugated (bs-10580R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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