Anti-JMJD4 antibody (bs-17205R)

Anti-JMJD4 antibody

DATASHEET

Host:Rabbit

Target Protein:JMJD4

IR:Immunogen Range:381-463/463

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:65049

Swiss Prot:Q9H9V9

Source:KLH conjugated synthetic peptide derived from human JMJD4:381-463/463 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:A crucial regulator of chromatin dynamics and DNA transcription is the covalent modification and methylation of histones. Generally, methylation of certain lysine residues on Histone H3 and Histone H4 can be associated with transcriptionally active or inactive chromatin. This regulation has profound effects on the expression of genes and is part of an epigenetic memory network that determines cell fate. JMJD4 (Jumonji domain-containing protein 4) is a member of a family of JMJC domain-containing histone demethylases that are directly involved in removing methyl residues from distinct and unique lysine residues. These actions are implicated in gene expression and the regulation of cell senescence. JMJC domain-containing histone demethylases are also likely involved in development via their ability to regulate gene expression.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
HepG2(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Panc-1(Human) Cell Lysate at 30 ug
Primary: Anti-JMJD4 (bs-17205R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD