Anti-phospho-SPHK2 (Thr614) antibody (bs-17661R)

Anti-phospho-SPHK2 (Thr614) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-SPHK2 (Thr614)

IR:Immunogen Range:PL(p-T)PR

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:56848

Swiss Prot:Q9NRA0

Source:KLH conjugated synthesised phosphopeptide derived from human SPHK2 around the phosphorylation site of Thr614:PL(p-T)PR 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Siha(huamn) Cell Lysate at 40 ug
Primary: Anti-p-SPHK2(Thr614) (bs-17661R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 75 kD