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Anti-phospho-MNK1 (Thr250) antibody (bs-17696R)

Anti-phospho-MNK1 (Thr250) antibody (bs-17696R)

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Anti-phospho-MNK1 (Thr250) antibody

英文名称phospho-MNK1 (Thr250)
中文名称磷酸化MAP激酶相互作用丝氨酸/苏氨酸激酶1抗体
别    名MNK1 (phospho T250); p-MNK1 (phospho T250); MAP kinase interacting kinase 1; MAP kinase interacting serine/threonine kinase 1; MAP kinase signal integrating kinase 1; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal integrating kinase 1; MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1; MKNK1; MKNK1_HUMAN; MNK 1; Mnk1.  

DATASHEET

Host:Rabbit

Target Protein:phospho-MNK1 (Thr250)

IR:Immunogen Range:IT(p-T)PE

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:8569

Swiss Prot:Q9BUB5

Source:KLH conjugated synthesised phosphopeptide derived from human MNK1 around the phosphorylation site of Thr250:IT(p-T)PE 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]

Size:100ul

Concentration:1mg/ml

Applications:IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MNK1 (Thr250)) Polyclonal Antibody, Unconjugated (bs-17696R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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