Anti-MSTO1 antibody
英文名称 | MSTO1 |
中文名称 | MSTO1蛋白抗体 |
别 名 | DKFZp686B1757; DKFZp686I01261; FLJ10504; LST005; misato homolog 1 (Drosophila); MST; Protein misato homolog 1; RP11 29H23.3. |
DATASHEET
Host:Rabbit
Target Protein:MSTO1
IR:Immunogen Range:151-250/570
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:55154
Swiss Prot:Q9BUK6
Source:KLH conjugated synthetic peptide derived from human MSTO1:151-250/570
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Drosophila melanogaster is a proven and effective model for studying developmental and cellular processes common to higher eukaryotes. Approximately 13,600 genes have been elucidated from more than 120 megabases of euchromatin, and they are organized among the chromosomes 2, 3, 4, X and Y, with the Y chromosome being predominately heterochromatic (1). Drosophila genes can be categorized based on the type of protein for which they encode and are represented by six major classifications, which include intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing, and chromatin associated) or other functional proteins (2). The misato gene encodes a protein that contains a mixture of peptide motifs found in alpha-, beta-, and gamma-tubulins, as well as a motif related to part of the myosin heavy chain proteins (3-5). Null mutations at the misato locus of Drosophila melanogaster are associated with irregular chromosomal segregation at cell division and result in larvae that have reduced levels of imaginal disk tissue, a reduction in brain size, and die during the larval stage of development (4,5).
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MSTO1) Polyclonal Antibody, Unconjugated (bs-17863R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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