Anti-SAKS1/2B28 antibody (bs-18331R)

Anti-SAKS1/2B28 antibody

DATASHEET

Host:Rabbit

Target Protein:SAKS1/2B28

IR:Immunogen Range:51-150/297

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:51035

Swiss Prot:Q04323

Source:KLH conjugated synthetic peptide derived from human SAKS1/2B28:51-150/297 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:2B28 is a component of a complex composed of NGLY1, SAKS1, AMFR, VCP and RAD23B, where it acts as an adapter that directs VCP to polyubiquitinated proteins. 2B28 also interacts with Homer 2, which further suggests role for 2B28 in the regulation of protein degradation by ubiquitin-proteasome systems.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SAKS1 2B28) Polyclonal Antibody, Unconjugated (bs-18331R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.