Anti-LRRC20 antibody (bs-18370R)

Anti-LRRC20 antibody

DATASHEET

Host:Rabbit

Target Protein:LRRC20

IR:Immunogen Range:51-150/184

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:55222

Swiss Prot:Q8TCA0

Source:KLH conjugated synthetic peptide derived from human LRRC20:51-150/184 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:LRRC18 is a 261 amino acid protein that contains seven LRR repeats. Localized to the cytoplasm, LRRC18 may be involved in spermatogenesis and sperm maturation. LRRC18 is expressed as two isoforms produced by alternative splicing and is encoded by a gene mapping to human chromosome 10.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRRC20) Polyclonal Antibody, Unconjugated (bs-18370R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.