Anti-MAN2C1 antibody (bs-18649R)

Anti-MAN2C1 antibody 

DATASHEET

Host:Rabbit

Target Protein:MAN2C1

IR:Immunogen Range:61-160/1040

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4123

Swiss Prot:Q9NTJ4

Source:KLH conjugated synthetic peptide derived from human MAN2C1:61-160/1040 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Misfolded glycoproteins are deglycosylated by the peptide N-glycanase during the degradation process. Free oligosaccharides released by N-glycanase are catabolized by cytosolic MAN2C1, also designated alpha-mannosidase 2C1. MAN2C1, a member of the glycosyl hydrolase 38 family, can cleave alpha 1,2-linked, alpha 1,3-linked and alpha 1,6-linked mannose residues and is stimulated by cobalt. The furanose analogs, swainsonine (SW) and 1,4-dideoJNC-1,4-imino-d-mannitol (DIM), are known inhibitors of MAN2C1. The inhibition of MAN2C1 can enhance the adhesion of Jurkat T cells, showing a cytoskeletal rearrangement of the cells.

Size:100ul

Concentration:1mg/ml

Applications:IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAN2C1) Polyclonal Antibody, Unconjugated (bs-18649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.