Anti-NARS2 antibody (bs-19024R)

Anti-NARS2 antibody 

DATASHEET

Host:Rabbit

Target Protein:NARS2

IR:Immunogen Range:101-200/477

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:79731

Swiss Prot:Q96I59

Source:KLH conjugated synthetic peptide derived from human NARS2:101-200/477 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:SLM5 (mitochondrial asparaginyl-tRNA synthetase, Asparagine-tRNA ligase) is a mitochondrial protein encoded by the human gene NARS2. AsnRS belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl tRNA synthetases (aaRS) are enzymes that catalyze the esterification of a specific amino acid or its precursor to its compatible cognate tRNA to form an aminoacyl-tRNA. The synthetase first binds ATP and the corresponding amino acid or its precursor to form an aminoacyl-adenylate and release inorganic pyrophosphate (PPi). The adenylate-aaRS complex then binds the appropriate tRNA molecule, and the amino acid is transferred from the aa-AMP to either the 2'- or 3'-OH of the last tRNA base (A76) at the 3'-end. Some synthetases also mediate a proofreading reaction to ensure high fidelity of tRNA charging; if the tRNA is found to be improperly charged, the aminoacyl-tRNA bond is hydrolyzed. SLM5 acts to attach asparagine residues to its cognate tRNA.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARS2) Polyclonal Antibody, Unconjugated (bs-19024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.