Anti-CHKL antibody (bs-10935R)

Anti-CHKL antibody 

DATASHEET

Host:Rabbit

Target Protein:CHKL

IR:Immunogen Range:301-395/395

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1120

Swiss Prot:Q9Y259

Source:KLH conjugated synthetic peptide derived from human CHKL:301-395/395 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Choline kinase (CK) and ethanolamine kinase (EK) catalyzethe phosphorylation of choline/ethanolamine tophosphocholine/phosphoethanolamine. This is the first enzyme in thebiosynthesis of phosphatidylcholine/phosphatidylethanolamine in allanimal cells. The highly purified CKs from mammalian sources andtheir recombinant gene products have been shown to have EK activityalso, indicating that both activities reside on the same protein.The choline kinase-like protein encoded by CHKL belongs to thecholine/ethanolamine kinase family; however, its exact function isnot known. Read-through transcripts are expressed from this locusthat include exons from the downstream CPT1B locus. [provided byRefSeq, Jun 2009].

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CHKL Polyclonal Antibody, Unconjugated(bs-10935R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining