Anti-Phospho-Paxillin (Ser83) antibody (bs-10992R)

Anti-Phospho-Paxillin (Ser83) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-Paxillin (Ser83)

IR:Immunogen Range:PQ(p-S)SS

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5829

Swiss Prot:P49023

Source:KLH conjugated Synthesised phosphopeptide derived from human Paxillin around the phosphorylation site of Ser83:PQ(p-S)SS 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-Paxillin (Ser83) Polyclonal Antibody, Unconjugated(bs-10992R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining