Anti-PRSS35 antibody (bs-19440R)

Anti-PRSS35 antibody

DATASHEET

Host:Rabbit

Target Protein:PRSS35

IR:Immunogen Range:121-220/413

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:167681

Swiss Prot:Q8N3Z0

Source:KLH conjugated synthetic peptide derived from human PRSS35:121-220/413 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:PRSS35, although related to the peptidase S1 family, lacks the conserved active Ser residue in position 346 which is replaced by a Thr, suggesting that it has no protease activity. This Ser to Thr substitution may play an important role in determining a unique pattern of substrate specificity for PRSS35. Alternatively, this substitution may yield an inactive protease that binds to specific substrates, thus protecting them from proteolysis. The Mouse Prss35 gene is selectively expressed in the ovary and it's expression prior to ovulation is, in part, regulated by progesterone. PRSS35 may be involved in ovulation and the formation and regression of the corpus luteum.

Size:100ul

Concentration:1mg/ml

Applications:IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRSS35) Polyclonal Antibody, Unconjugated (bs-19440R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.