Anti-WNT 5B antibody (bs-8010R)

Anti-WNT 5B antibody

DATASHEET

Host:Rabbit

Target Protein:WNT 5B

IR:Immunogen Range:201-300/359

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:81029

Swiss Prot:Q9H1J7

Source:KLH conjugated synthetic peptide derived from human WNT 5B:201-300/359 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Bladder (Mouse)Lysate at 40 ug
Primary: Anti- WNT 5B(bs-8010R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38kD

Sample:Bladder (Mouse)Lysate at 40 ug
Primary: Anti- WNT 5B(bs-8010R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 38kD