Anti-HAP1 antibody (bs-20303R)

Anti-HAP1 antibody

DATASHEET

Host:Rabbit

Target Protein:HAP1

IR:Immunogen Range:551-650/671

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9001

Swiss Prot:P54257

Source:KLH conjugated synthetic peptide derived from human HAP1:551-650/671 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Huntingtin-associated protein 1 binds to Huntingtin. Huntingtin is a protein that contains a polyglutamine region and when the number of glutamine repeats exceeds 35, the gene encodes a version of Huntingtin that leads to Huntington’s disease (HD). The ability of HAP1 to bind to Huntingtin is enhanced by an expanded polyglutamine repeat region. HAP1 shows neuronal localization and moves with Huntingtin in nerve fibers. HAP1 is primarily expressed in brain tissue, with greater expression in the olfactory bulb and brain stem. HAP1 in rat has been shown to associate with a number of intracellular organelles. Mouse HAP1 is localized to membrane-bound organelles including large endosomes, tubulovesicular structures and budding vesicles in neurons.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HAP1) Polyclonal Antibody, Unconjugated (bs-20303R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.