Anti-RCAS1/EBAG9 antibody
| 英文名称 | RCAS1/EBAG9 |
| 中文名称 | 肿瘤相关表面抗原EBAG9抗体 |
| 别 名 | BAG9; Cancer associated surface antigen; Cancer associated surface antigen RCAS1; EB9; EBAG 9; Estrogen receptor binding fragment associated gene 9 protein; Estrogen receptor binding site associated antigen 9; PDAF; RCAS 1; RCAS1; Receptor binding cancer antigen expressed on SiSo cells. |
DATASHEET
Host:Rabbit
Target Protein:RCAS1/EBAG9
IR:Immunogen Range:151-213/213
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9166
Swiss Prot:O00559
Source:KLH conjugated synthetic peptide derived from human RCAS1:151-213/213
Purification:affinity purified by Protein A
Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Chicken
Pig
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: Mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RCAS1 Polyclonal Antibody, Unconjugated(bs-10982R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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