Anti-Dystrobrevin alpha antibody (bs-23134R)

Anti-Dystrobrevin alpha antibody

DATASHEET

Host:Rabbit

Target Protein:Dystrobrevin alpha

IR:Immunogen Range:301-400/743

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:13527

Swiss Prot:Q9D2N4

Source:KLH conjugated synthetic peptide derived from mouse Dystrobrevin alpha:301-400/743 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Dystrobrevin alpha belongs to the dystrobrevin subfamily of the dystrophin family. It is a component of the dystrophin associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha and beta dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin alpha may be involved in the formation and stability of synapses as well as being involved in the clustering of nicotinic acetylcholine receptors. Mutations in Dystrobrevin alpha are associated with left ventricular noncompaction with congenital heart defects.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Dog
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dystrobrevin alpha) Polyclonal Antibody, Unconjugated (bs-23134R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.