Anti-CD146 antibody (bs-20677R)

Anti-CD146 antibody

DATASHEET

Host:Rabbit

Target Protein:CD146

IR:Immunogen Range:201-300/646

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4162

Swiss Prot:P43121

Source:KLH conjugated synthetic peptide derived from human CD146:201-300/646 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:MCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion.

Size:100ul

Concentration:1mg/ml

Applications:Flow-Cyt(1ug/test)
ICC(1:100-500)

Cross Reactive Species:Human
Mouse
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control:U-2OS.
Primary Antibody (green line): Rabbit Anti-CD146 antibody (bs-20677R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.