Anti-PYCR1 antibody (bs-20864R)

Anti-PYCR1 antibody

DATASHEET

Host:Rabbit

Target Protein:PYCR1

IR:Immunogen Range:241-309/309

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:209027

Swiss Prot:Q922W5

Source:KLH conjugated synthetic peptide derived from mouse PYCR1:241-309/309 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboJNClate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PYCR1) Polyclonal Antibody, Unconjugated (bs-20864R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.