Anti-LDHB antibody (bs-20294R)

Anti-LDHB antibody

DATASHEET

Host:Rabbit

Target Protein:LDHB

IR:Immunogen Range:181-250/334

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3945

Swiss Prot:P07195

Source:KLH conjugated synthetic peptide derived from human LDHB:181-250/334 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Recent studies have shown that two protein isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism, and that these C-terminally distinct isoforms have different subcellular localization. Alternatively spliced transcript variants have also been found for this gene. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Oct 2015]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Heart (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Jurkat (Human) Lysate at 40 ug
Primary: Anti-LDHB (bs-20294R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD