Anti-VP16 tag antibody (bs-2111R)

Anti-VP16 tag antibody

DATASHEET

Host:Rabbit

Target Protein:VP16 tag

IR:Immunogen Range:391-490/490aa

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:VP16 tag

Swiss Prot:P04486

Source:KLH conjugated synthetic peptide derived from HSV-1 VP16:391-490/490aa 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:HSV evades the immune system through interference with MHC class I antigen presentation on the cell surface, by blocking TAP or the transporter associated with antigen processing induced by the secretion of ICP-47 by HSV. In the host cell, TAP transports digested viral antigen epitope peptides from the cytosol to the endoplasmic reticulum, allowing these epitopes to be combined with MHC class I molecules and presented on the surface of the cell. Viral epitope presentation with MHC class I is a requirement for activation of cytotoxic T-lymphocytes (CTLs), the major effectors of the cell-mediated immune response against virally-infected cells. ICP-47 prevents initiation of a CTL-response against HSV, allowing the virus to survive for a protracted period in the host.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:1000-5000)
ELISA(1:5000-10000)

Cross Reactive Species:Human herpesvirus 1,Fusion Protein.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: VP16-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: VP16-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-VP16 tag (bs-2111R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD