Anti-AMPK gamma 1 antibody (bs-23404R)

Anti-AMPK gamma 1 antibody

DATASHEET

Host:Rabbit

Target Protein:AMPK gamma 1

IR:Immunogen Range:1-100/559

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5571

Swiss Prot:P54619

Source:KLH conjugated synthetic peptide derived from human AMPK gamma 1:1-100/559 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The 5 AMP activated protein kinase (AMPK), a member of the SNF1 (sucrose non fermentor) kinase family, is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroJNCmethylglutaryl CoA reductase. AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMPK gamma 1) Polyclonal Antibody, Unconjugated (bs-23404R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.