Anti-Melatonin Receptor 1A antibody (bs-21739R)

Anti-Melatonin Receptor 1A antibody

DATASHEET

Host:Rabbit

Target Protein:Melatonin Receptor 1A

IR:Immunogen Range:181-280/350

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4543

Swiss Prot:P48039

Source:KLH conjugated synthetic peptide derived from human Melatonin Receptor 1A :181-280/350 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1ug/Test)

Cross Reactive Species:Human
Mouse
Rat
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control:Mouse brain.
Primary Antibody (green line): Rabbit Anti-Melatonin Receptor 1A antibody (bs-21739R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.