Anti-EDG8 antibody
| 英文名称 | EDG8 |
| 中文名称 | 内皮细胞分化G蛋白偶联受体8抗体 |
| 别 名 | EDG 8; Endothelial differentiation G protein coupled receptor 8; Endothelial differentiation G-protein-coupled receptor 8; Endothelial Differentiation Gene 8; Endothelial differentiation sphingolipid G protein coupled receptor 8; S1P receptor 5; S1P receptor Edg 8; S1P receptor Edg-8; S1P receptor Edg8; S1P5; S1PR5; S1PR5_HUMAN; Sphingosine 1 Phosphate Receptor 5; Sphingosine 1 phosphate receptor Edg 8; Sphingosine 1 phosphate receptor Edg8; Sphingosine 1-phosphate receptor 5; Sphingosine 1-phosphate receptor Edg-8; SPPR 1; SPPR 2; SPPR1; SPPR2. |
DATASHEET
Host:Rabbit
Target Protein:EDG8
IR:Immunogen Range:1-100/398
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:53637
Swiss Prot:Q9H228
Source:KLH conjugated synthetic peptide derived from human EDG8 :1-100/398
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. EDG-6, which is expressed in lymphoid and hematopoietic tissues and in lung, signals through Gi/o proteins, which activate growth related pathways.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDG8) Polyclonal Antibody, Unconjugated (bs-21807R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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