Anti-PTF1A antibody (bs-22058R)

Anti-PTF1A antibody 

DATASHEET

Host:Rabbit

Target Protein:PTF1A

IR:Immunogen Range:141-240/328

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:256297

Swiss Prot:Q7RTS3

Source:KLH conjugated synthetic peptide derived from human PTF1A:141-240/328 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:PTF1 is a pancreas-specific protein that functions as a component of the trimeric pancreas transcription factor 1 (PTF1) complex. Localizing to the nucleus, PTF1 contains one basic helix-loop-helix (bHLH) domain and is believed to play an important role in mammalian pancreatic development, functioning as a transcription factor that regulates the specification of all three pancreatic cell types. PTF1 interacts with RBP-Jⅹ and, together, they cooperate in regulating the expression of PDX-1 (pancreas/duodenum homeobox protein 1), a key regulator of pancreatic islet development and insulin gene transcription in beta-cells. Loss of functional PTF1 can cause pancreatic progenitors to take on the normal fates of duodenal epithelia. Mutations in the gene encoding PTF1 lead to diabetes mellitus and cerebellar hypoplasia/agenesis, suggesting that PTF1 also plays and important role in cerebellar neurogenesis.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Pancreas (Mouse) Lysate at 40 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti- PTF1A (bs-22058R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 38 kD