Anti-Cytochrome C antibody (bs-22900R)

Anti-Cytochrome C antibody

DATASHEET

Host:Rabbit

Target Protein:Cytochrome C

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:54205

Swiss Prot:P99999

Source:Cytochrome c from bovine heart : 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Mouse
Rat
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Heart (Rat) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome C (bs-22900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14.4 kD
Observed band size: 14.4 kD