Anti-Lamin B antibody (bs-24328R)

Anti-Lamin B antibody

DATASHEET

Host:Rabbit

Target Protein:Lamin B

IR:Immunogen Range:381-480/586

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4001

Swiss Prot:P20700

Source:KLH conjugated synthetic peptide derived from human Lamin B:381-480/586 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: U937 (Human) Cell Lysate at 30 ug
Lane 2: Jurkat (Human) Cell Lysate at 30 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: U2os (Human) Cell Lysate at 30 ug
Primary: Anti-Lamin B (bs-24328R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67-70 kD
Observed band size: 67 kD