Anti-MGMT antibody (bs-24479R)

Anti-MGMT antibody

DATASHEET

Host:Rabbit

Target Protein:MGMT

IR:Immunogen Range:1-100/207

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:17314

Swiss Prot:P26187

Source:KLH conjugated synthetic peptide derived from mouse MGMT:1-100/207 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine, and other methylated moities of DNA, to a cysteine residue in itself, thus repairing DNA damage caused by alkylation in a single step reaction. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT prevents mutagenesis and malignant transformation, and also provides resistance to chemotherapy with alkylating agents among cancer patients.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Lymph node (Rat) Lysate at 40 ug
Lane 2: Small intestine (Rat) Lysate at 40 ug
Primary:
Anti-MGMT (bs-24479R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD