Anti-Histone H3 (acetyl K4) antibody (bs-24644R)

Anti-Histone H3 (acetyl K4) antibody

DATASHEET

Host:Rabbit

Target Protein:Histone H3 (acetyl K4)

IR:Immunogen Range:RT(Acetyl-K)QT

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:8290

Swiss Prot:Q16695

Source:KLH conjugated synthetic peptide derived from human Histone H3 (acetyl K4):RT(Acetyl-K)QT 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Primary: Anti-Histone H3 (acetyl K4) (bs-24644R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 17 kD