Anti-Vesicular Acetylcholine Transporter antibody (bs-1833R)

Anti-Vesicular Acetylcholine Transporter antibody

DATASHEET

Host:Rabbit

Target Protein:Vesicular Acetylcholine Transporter

IR:Immunogen Range:201-300/532

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6572

Swiss Prot:Q99536

Source:KLH conjugated synthetic peptide derived from human VAT:201-300/532 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Vesicular Acetylcholine Transporter (VAChT), (~70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate.
VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer’s disease and other nervous system disorders.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.