Anti-Phospho-Gab1 (Tyr627) antibody (bs-3165R)

Anti-Phospho-Gab1 (Tyr627) antibody 

DATASHEET

Host:Rabbit

Target Protein:Phospho-Gab1 (Tyr627)

IR:Immunogen Range:VE(p-Y)LD

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2549

Swiss Prot:Q13480

Source:KLH conjugated Synthesised phosphopeptide derived from human Gab1 around the phosphorylation site of Tyr627:VE(p-Y)LD 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus, activating mitogenic as well as other responses. Critical components of this process include adapter proteins such as Shc, IRS-1 and Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity (1-3,8). Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.