Anti-AX2R antibody (bs-7669R)

Anti-AX2R antibody

DATASHEET

Host:Rabbit

Target Protein:AX2R

IR:Immunogen Range:30-90/193

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:389289

Swiss Prot:Q3ZCQ2

Source:KLH conjugated synthetic peptide derived from human AX2R:30-90/193 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:AX2R protein may act as a receptor for annexin II on marrow stromal cells to induce osteoclast formation.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control (Black line): U937(Black).
Primary Antibody (green line): Rabbit Anti-AX2R Conjugated FITC antibody (bs-7669R-FITC)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA(10min at room temperature). The cells were then incubated in 5% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was perf