Anti-IQCD antibody
| 英文名称 | IQCD |
| 中文名称 | IQCD蛋白抗体 |
| 别 名 | IQ domain containing protein D; IQ domain-containing protein D; IQ motif containing D; IQCD; IQCD_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:IQCD
IR:Immunogen Range:201-300/449
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:115811
Swiss Prot:Q96DY2
Source:KLH conjugated synthetic peptide derived from human IQCD:201-300/449
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-IQCD antibody (bs-9017R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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