Anti-LGR6 antibody (bs-8609R)

Anti-LGR6 antibody

DATASHEET

Host:Rabbit

Target Protein:LGR6

IR:Immunogen Range:401-500/967

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:59352

Swiss Prot:Q9HBX8

Source:KLH conjugated synthetic peptide derived from human LGR6:401-500/967 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors and heptahelical receptors, are a protein family which interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. The GPCR family represents the largest class of targets for modern drugs. Leucine-rich repeat-containing G protein-coupled receptor 6 (LGR6) is an orphan-A GPCR with an N-terminal ectodomain comprising variable leucine-rich repeats, a 7TM region and a unique C-terminal intracellular tail. The sequence of LGR6 is similar to TSHR, FSHR and LHR. LGR6 is predominantly expres-sed in the adrenal gland, ovary and uterus.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-LGR6 antibody (bs-8609R-AF488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.