Anti-phospho-MRCL3 (Thr18 + Ser19) antibody (bs-17750R)

Anti-phospho-MRCL3 (Thr18 + Ser19) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-MRCL3 (Thr18 + Ser19)

IR:Immunogen Range:RA(p-T)(p-S)N

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10627

Swiss Prot:P19105

Source:KLH conjugated synthesised phosphopeptide derived from human MRCL3 around the phosphorylation site of Thr18 + Ser19:RA(p-T)(p-S)N 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Myosin regulatory light chains, including MRCL3 (also known as MRLC3 or MLCB), MRLC2 (also known as MLC-B) and MYL9 (also known as LC20, MLC2, MRLC1 or MYRL2), regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the Actin-activated myosin ATPase activity, thereby regulating the contractile activity. Myosin light chain is also located in striated skeletal muscle, where its function remains undefined.

Size:100ul

Concentration:1mg/ml

Applications:IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Zebrafish
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.