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Anti-NPTII/Neomycin Phosphotransferase 2 antibody (bs-19203R)

Anti-NPTII/Neomycin Phosphotransferase 2 antibody (bs-19203R)

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Anti-NPTII/Neomycin Phosphotransferase 2 antibody

英文名称NPTII/Neomycin Phosphotransferase 2
中文名称肺炎克雷伯菌新霉素磷酸转移酶2抗体
别    名Klebsiella pneumoniae Aminoglycoside 3' phosphotransferase; APH(3') II; APH(3')II; aphA; EC 2.7.1.95; Kan; Kanamycin kinase type II; Kanamycin kinase, type VIII; kanamycin resistance gene; Neo; Neomycin kanamycin phosphotransferase type II; Neomycin kanamycin phosphotransferase type VIII; Neomycin phosphotransferase II; Neomycine phosphotransferase; NPT II; NPTII; KKA2_KLEPN.

DATASHEET

Host:Rabbit

Target Protein:NPTII/Neomycin Phosphotransferase 2

IR:Immunogen Range:101-200/264

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:N/A

Swiss Prot:P00552

Source:KLH conjugated synthetic peptide derived from Klebsiella pneumoniae Neomycin Phosphotransferase 2:101-200/264 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin? G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Klebsiella pneumoniae.

For research use only. Not intended for diagnostic or therapeutic use.

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